Purifies dna from whole blood samples using the maxwell rsc instrument. Using one simple protocol, a high yield of purified dna can be isolated from a wide variety of sources including whole blood fresh or frozen, citrate, heparin or edtatreated, buffy coat, leukocytes, milk, seminal fluid, dried blood spots, cultured cells, tissue fresh, frozen or. The isolated dna is 2050 kb in size and is suitable fo r pcr, restriction enzyme digestion, and southern blotting. This protocol is designed for purification of total dna from grampositive bacteria. C, and grampositive and gramnegative bacteria section 3. Dnaland scientific genomic dna isolation kit uses silicagelmembrane technology for simple and fast isolation of genomic dna without phenol. Dna isolated with the dna extraction kit is free from contaminants and may be used directly for restriction digests, cloning, southern blotting, pcr amplification, and other dna analysis techniques. Table 1 lists the recommended starting material from each of these sources.
The extracted and purified dna is suitable for any molecular biology procedure, including but not limited to pcr polymerase chain. Proteins and other contaminants are eliminated through a. Each grain was soaked with clean dw for overnight and. Highthroughput extraction of gdna from a range of sample types in 24 or 96well format. The kit provides a rapid method for the isolation and purification of total rna and dna sequentially from a single sample of cultured animal cells and tissues, blood, bacteria, yeast, or fungi. In this study, we compared commercial and noncommercial dna extraction protocols. Optimized combination of extraction kit and pcr assay biotecon diagnostics has developed a broad range of fast, safe and easytouse dna and rna extraction kits for any food, beverage or. High yields of plant genomic dna genomic dna was isolated from 20 mg freezedried. Reliaprep blood gdna miniprep system dna extraction kit. Genomic dna isolation kit for blood, bacteria, mouse tail. Dna from whole blood, plasma, or other body fluids. The first step in the purification procedure lyses the cells and the. Qiaamp dna micro kit qiagen, stanford, ca, usa, qiaamp dna mini kit qiagen, mem.
Instruction manual monarch genomic dna purification kit. Purification of total rna from bacteria using the rneasy. The tacotm dnarna extraction kit has to be used with the. Comparison of dna extraction methods from small samples of. The wizard genomic dna purification kit is designed for isolation of dna from white blood cells, tissue culture cells and animal tissue,plant tissue, yeast, grampositive and gramnegative bacteria. The tissue and hair extraction kit protocol includes details for purification of genomic and mitochondrial dna from hair follicles and hair shafts and purification of genomic dna from fresh, frozen, or formalin. The protocol works with the following anticoagulants. This kit uses a specially treated glass filter membrane. Gram negative bacterial cell lysate protocol purelink. Purify genomic dna from human blood samples using the iprep purification instrument as described below.
Wizard genomic dna purification kit protocol promega. Maxwell 16 tissue dna purification kit promega corporation. With this system alone, chromosomal dna can be isolated from whole blood 5, plant leaf 6, grampositive 7 and gramnegative bacteria 8, mouse tail 9 and yeast 10. Up to 1 x 10 9 bacteria are disrupted and homogenized by beadmilling in a guanidinethiocyanatecontaining lysis buffer. How dna extraction kits work in the lab bitesize bio. Dna purified with this system is suited for longread sequencing platforms. Purification of total dna from plant tissue mini protocol important points before starting if using the dneasy plant mini kit for the first time, read important notes page 16.
The final step in the dna extraction protocol is the release of pure dna or rna from the silica. Thermo scientific genomic dna purification kit is a simple and rapid system for high quality genomic dna purification from various sources including. Dna is ready for pcr, realtime pcr, southern blotting and rflp. Tissue and hair extraction kit for use with dna iq protocol. Tissue and hair extraction kit for use with dna iq cat.
Maxwell 16 dna purification kits technical manualpdf 487 kb english. Specific dna extraction methods, which are optimized for a variety of sample matrices, are the prerequisite for the success of subsequent analysis methods. System overview the purelink genomic dna mini kit is based on the selective bindin g of. We highly recommend the use of gloves and aerosolresistant pipette tips. The wizard hmw dna extraction kit is designed for isolation of highmolecularweight dna from white blood cells section 3. The wizard genomic dna purification kit is based on a fourstep process. Product components and storage conditions smallscale isolation minipreps product size cat. For details on using the iprep purification instrument, refer to the manual supplied with the. A protocol for isolation of genomic dna from white blood cells, tissue culture cells, animal and plant tissue, yeast, grampositive and gramnegative. Geneproof pathogenfree dna isolation kit are designed for the rapid isolation of highly pure genomic.